Journal: Cells

Article Title: Establishment and Characterization of Cell Lines from Canine Metastatic Osteosarcoma

doi: 10.3390/cells13010025

Figure Lengend Snippet: Cell doubling time for three cell lines and osteoblasts.

Article Snippet: Two osteoblast cell strains were established from pieces of rib bone from 13-week-old beagles at euthanasia, carried out for reasons unrelated to this study, and were cultured in the lab. One commercially available strain of primary canine osteoblasts was also used (Cell Applications, San Diego, CA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential

doi: 10.3390/ijms22168764

Figure Lengend Snippet: Growth curves of HOB-01-C1 cells treated with different PRP dilutions, obtained by Resazurin assay: cells seeded in complete medium containing 10% HSAB (Positive control, red curve); 1% PRP (magenta); 10% PRP (green); 25% PRP (blue), and 50% PRP (black). The X -axis reports the days of culture, whereas the Y -axis reports the relative fluorescence units (RFU). Data are expressed as mean ± SD of three independent experiments.

Article Snippet: HOB-01-C1 cells were seeded at a density of 500 cells/well in 96-well plates (EUROCLONE SpA, Milan, Italy) in Dulbecco’s Modified Eagle Medium (DMEM) Low Glucose (EUROCLONE SpA, Milan, Italy) supplemented with 1% fetal bovine serum (EUROCLONE SpA, Milan, Italy), 200 mM Glutamine and 1% Streptomycin/Penicillin (both from Sigma-Aldrich s.r.l., Milan, Italy).

Techniques: Resazurin Assay, Positive Control, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential

doi: 10.3390/ijms22168764

Figure Lengend Snippet: Calcium deposition in the extracellular matrix induced by PRP treatment. ( a ) Optical microscopy images of HOB-01-C1 cells treated with different PRP dilutions (1–50%) and stained at T14 with Alizarin Red S (10×) Scale bar 100 μm; ( b ) Alizarin Red S staining quantification with different PRP dilutions at T14. Data are reported as fold change vs. negative control and expressed as mean ± SD of three independent experiments. Statistical analysis was performed by one-way ANOVA test (* p < 0.05; ** p < 0.01).

Article Snippet: HOB-01-C1 cells were seeded at a density of 500 cells/well in 96-well plates (EUROCLONE SpA, Milan, Italy) in Dulbecco’s Modified Eagle Medium (DMEM) Low Glucose (EUROCLONE SpA, Milan, Italy) supplemented with 1% fetal bovine serum (EUROCLONE SpA, Milan, Italy), 200 mM Glutamine and 1% Streptomycin/Penicillin (both from Sigma-Aldrich s.r.l., Milan, Italy).

Techniques: Microscopy, Staining, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential

doi: 10.3390/ijms22168764

Figure Lengend Snippet: Calcein Green fluorescence of HOB-01-C1 cells treated with 1–50% PRP dilutions: ( a ) fluorescence microscopy images (10×) of PRP Sample 3 (chosen as a representative example) stained after 12 days of culture (T12) Scale bar 100 μm; ( b ) Semi-quantitative analysis, acquired at T12. Data are expressed as mean fluorescence ± SD of three independent experiments. (* p < 0.05; ** p < 0.001).

Article Snippet: HOB-01-C1 cells were seeded at a density of 500 cells/well in 96-well plates (EUROCLONE SpA, Milan, Italy) in Dulbecco’s Modified Eagle Medium (DMEM) Low Glucose (EUROCLONE SpA, Milan, Italy) supplemented with 1% fetal bovine serum (EUROCLONE SpA, Milan, Italy), 200 mM Glutamine and 1% Streptomycin/Penicillin (both from Sigma-Aldrich s.r.l., Milan, Italy).

Techniques: Fluorescence, Microscopy, Staining

Journal: International Journal of Molecular Sciences

Article Title: Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential

doi: 10.3390/ijms22168764

Figure Lengend Snippet: Gene expression of HOB-01-C1 cells at a day 12 after seeding (T12) and treated with different dilutions of PRP (1–25%). ( a ) RUNX2; ( b ) COL1A1; ( c ) OCN; ( d ) PHEX. Each gene was normalized for Ribosomal Protein 15 (RPL15). Data are reported as fold of expression (Fold change) vs. Negative Control and represented as mean ± SD of three independent experiments performed on sample 4. Statistical analysis was performed by one-way Anova. (* p < 0.05; ** p < 0.01; *** p < 0.001).

Article Snippet: HOB-01-C1 cells were seeded at a density of 500 cells/well in 96-well plates (EUROCLONE SpA, Milan, Italy) in Dulbecco’s Modified Eagle Medium (DMEM) Low Glucose (EUROCLONE SpA, Milan, Italy) supplemented with 1% fetal bovine serum (EUROCLONE SpA, Milan, Italy), 200 mM Glutamine and 1% Streptomycin/Penicillin (both from Sigma-Aldrich s.r.l., Milan, Italy).

Techniques: Expressing, Negative Control

Journal: Biomedicines

Article Title: Vertical Vibration of Mouse Osteoblasts Promotes Cellular Differentiation and Cell Cycle Progression and Induces Aging In Vitro.

doi: 10.3390/biomedicines11020444

Figure Lengend Snippet: Figure 6. Cellular aging resulting from the vibration of osteoblasts. (A,B): Real-time PCR, (C): Senescence-associated beta-galactosidase (SA-β-Gal) assay, (D): Telomere length assay (qPCR) (A): On applying vibrations at 30 Hz, aging-related genes (p53, p21, and p16) were significantly upregulated. (B): A stress-related gene (c-fos) was significantly upregulated at 30 Hz. (C): SA-β-Gal activity, an aging marker, was significantly upregulated at 30 Hz and 300 Hz. (D): Telomere length was significantly reduced at 30 Hz and 300 Hz. Dunnet’s multiple comparison test for 30 Hz and 300 Hz to 0 Hz (*: p < 0.05, **: p < 0.01).

Article Snippet: Primary osteoblasts (POB cells) were isolated from the maxillary bone of 8-weekold male mice (C57BL/6J, Charles River, Wilmington, MA, USA).

Techniques: Real-time Polymerase Chain Reaction, Activity Assay, Marker, Comparison